Author: Eshchanov, Khushnudbek Odilbekovich;Yakubov, Gayrat Kuvandikovich; Nurullayeva, Manzura Shavkatovna; Zaripova, Oydin

Annotation: In this article, the use of natural dyes and the creation of methods for their effective extraction, as well as the extraction of indigo dye from the Indigofera tinctoria L plant traditionally grown in the Khorezm region during our research, and the indigo dye particles in solution after product extraction options are provided. Extraction of indoxyl-β-D-glucoside from the leaves of Indigofera tinctoria L plant was carried out in aqueous medium, and hydrolysis of indoxyl-β-D-glucoside released into the solution was carried out with the presence of β-glucosidase enzyme. Lactobacillus acidophilus bacteria were used to obtain β-glucosidase enzyme. According to the obtained results, the formation of dye was observed in the remaining solution even after the indigo dye was extracted. In order to solve these problems, we conducted research.

Keywords: Indigofera tinctoria L, indigo, solution, indican, β-glucosidase, extraction, Lactobacillus acidophilus, indoxyl.

Pages in journal: 924 - 928